— Each individual cell-adhered micro-pattern within an e.g. 384-wellplate is independently monitored at fine temporal resolution over extended periods to directly observe the full range of contractile behavior, from tonic contraction to induced constriction or relaxation, and the action window of either effect. ‘X’ – shaped micro-patterns are chosen to minimize cell-substrate contact area, while maximizing cell-spread area, leading to amplified and concentrated force production at distinct locations. Since each micro-pattern is mechanically decoupled from the others, adhered cells do not transmit strain to neighboring micro-patterns, ensuring faithful assessment of contractile force for every single cell in the population.